Transplanting induced pancreatic islets from mono-clone human pancreatic stem cells for treatment diabetes in rats.

The monoclonal human pancreatic stem cells(about 1.0×104 cells/well) were cultured in 6-well plates.After proliferated for 3 days,these cells were induced to differentiate into pancreatic islets-like that included a lot of β cells.The induced functional pancreatic islets were positive with dithizone staining,expressed the transcription factor of the insulin by RT-PCR.Total thirty SpragueDawley adult rats were used for transplantation.Six rats as the negative control were individually injected with buffer.Twenty four rats were injected with 2% strptozotocin.On the 48 h,5 th and 8 th days after being injected with the STZ,blood samples were collected by cutting tail,and plasma glucose levels were measured.Rats with plasma glucose levels over 16.65 mmol/L by three time measurements were used as diabetes models for transplantation.The induced pancreatic islets invevery well which showed positive were suspended in 100 μL DMEM,and transplanted into the subcapsular region of the left kidney of twelve diabetes rats.At the same time,the subcapsular region of the left kidney of the other six diabetes model control rats was injected with 100 μL DMEM.The blood glucose levels of the experimental rats were measured in samples collected by cutting tail every 10 days.During 67 day experiment,the blood glucose levels of the twelve rats transplanted with induced pancreatic islets gradually decreased from 18.93-25.78 mmol/L to 6.32-11.47 mmol/L.These rats lived for 54-76 days,except two died 3 days by transplantation infection.Among six diabetes model control rats,five lived for 16-42 days with the blood glucose levels of 18.73-25.96 mmol/L and one died 3 days by transplantation infection.All six normal control rats were alive with the blood glucose levels of 3.54-5.50 mmol/L.These results indicated that the monoclonal human pan creaticstem cells could be in vitro induced into functional islet-like clusters included many β cells;The transplantation of induced pancreatic islets could decrease blood glucose levels of diabetes rats and prolong the life time.