Specific Protein Detection in Multiprotein Coexisting Environment by Using LSPR Biosensor

Specific protein detection in multiprotein coexisting environment by localized surface plasmon resonance (LSPR) biosensor is studied. After the surfaces of self-assembly fabricated triangular Ag nanoparticles on the chip are activated, a specific biological molecule layer with δ-toxic protein identifying function is linked to the chip. Based on the LSPR spectrometer measurement system in our laboratory, δ-toxic protein in sonicated Bacillus thuringiensis cell fractions (which consists of many kinds of proteins) is detected successfully by measuring the extinction spectrum of the chip.