Profiling Cytokine Levels Using a Multiplex Human Cytokine ELISA Array

3. The ELISA Array can also be used to determine the ratio of induction levels among multiple samples in one experiment by using a four-parameter logistic-log model. Experiments: Peripheral blood mononuclear cells (PBMC) purchased from AllCells were cultured in DMEM medium with 10% FBS, 1X MEM NEAA, 1X GlutaMaxTM-1, and 10 mM HEPES. PBMC were stimulated with 50 ng/ml PMA and 1 μg/ml ionomycin. Cell supernatants were collected at different time points (0, 6, 18, 24, and 48 hours) and the cytokine productions were measured by enzyme-linked immunosorbent assay (ELISA) using the Human Th1 / Th2 Cytokines MultiAnalyte Profiler ELISArrayTM Kit.