Efficient three-dimensional fluorescence measurements for characterization of binding properties in some plants

Abstract The main aim of this research was to characterize some plants and to determine their similarities and differences, using spectroscopic methods. The interactions of soluble polyphenols of different plants with human serum albumin (HSA) were investigated by 3D-fluorescence. The obtained fluorescence results allow to classify the investigated plants according to their binding properties. The HSA-binding capacities of these plants were the highest for asparagus and correlated with their antioxidant properties. To our knowledge this is the first report showing differences and similarities in these plants, using spectroscopic techniques. The fluorescence spectral methods, which were applied as a powerful tool showing the quenching properties of intrinsic fluorophores in protein molecules in the presence of some plant polyphenols, can contribute in pharmaceutical and food applications.