Validated Method Development for Estimation of Ketamine HCl as API and in Pharmaceutical Dosage Forms By UV-Spectroscopy.

Ketamine HCl is a white crystalline powder and anaesthetic drug. It is a noncompetitive NMDA receptor (NMDAR) antagonist. A method for the determination of Ketamine HCl in API and Pharmaceutical dosage form was developed in UV-Spectroscopy, which was found to be simple, sensitive, cost effective, reproducible, validated & economic. The maximum absorbance of Ketamine HCl was at 220 nm. The Beer’s Lambert law was obeyed successfully. The linearity was from 5 to 40µg/ml and effective range was found to be from 12 to 28 µg/ml in calibration curve. The correlation coefficient was found to be 0.999. The precision, accuracy, repeatability, specificity, linearity was done and all the parameters were found within the acceptable range. All the procedure were followed according to ICH guidelines.