무부하 자유수영 운동이 C57BL/6mouse 골격근의 PARP및 SIRT1 protein의 발현에 미치는 영향

The aim of this study was to investigate effect of PARP inhibition to SIRT1 expression and NAD cyclases during skeletal muscle contraction. unloading swimming exercise and PARP inhibitor, PJ34, used for PARP inhibition during muscle contraction in vivo and in vitro. NAD cyclase related protein expression level were compared by western blotting. Our results demonstrated that PARP inhibition not influenced to SIRT1 and PGC-1α expression. This means no co-regulation effect between SIRT1 and PARP. These results indicated that exercise induced muscle damage mediated PARP activating signal not involved exercise induced thermogenesis through SIRT1 activation.