16 THE METHODOLOGICAL ASPECTS OF PHOTODYNAMIC THERAPY WITH NEW PHOTOSENSITIZER OF CHLORIN LINE IN EXPERIMENT

2.0 2.5mg/kg. Cervix was irradiated superficially with light dose 50 J/cm2 (5 patients) or 100 J/cm2 in other 54 cases. Quality of ectocervix irradiation was monitored by the fluorescence imaging device. Endocervix was irradiated by fiberoptic light delivery system with cylindrical diffuser with active diffusive lengths of 2 3 cm and outer diameters of 1mm with light dose 100 300 J/cm of diffuser length. The PDT outcome was estimated every 3 month after treatment by the expanded colposcopy, proven by morphological and cytological study. HPV presence was detected by PCR (polymerase chain reaction) test. Results: After undergoing the PDT treatment 21 patients have been observed between 6 months and 1 year, 13 patients between 1 and 2 years and 25 patients for 2 years and more. Complete response to PDT, proven by cytological and histological study was observed in case of 53 patients (93%). Moderate skin photosensitivity was observed first 2 days after PDT as systematic side effect. No cervix deformation or scarring occurred due to PDT. HPV was detected in 52 out of 59 observed patients (88%). Followup at three months after PDT revealed that HPV was eradicated in 46 patients (80%). Conclusion: CIN-PDT with chlorin e6-polyvinylpyrrolidone formulation is an effective modality for the treatment of cervical intraepithelial neoplasia. CIN-PDT is effective not only in treating CIN but also for eradicating associated cervical HPV.