Evaluation of the combined treatment of ultraviolet light and peracetic acid as an alternative to chlorine washing for lettuce decontamination.

Abstract The potential of using ultraviolet light (UV) in combination of peracetic acid (PAA) as an alternative to chlorine washing for lettuce was evaluated. Shredded iceberg lettuce was dip-inoculated with a four-strain Salmonella cocktail to final levels of 6–7.5 log CFU/g, following by air-drying and overnight cold storage. The inoculated lettuce (80 g) was then washed in turbid tap water containing 6% lettuce juice extract and silicon dioxide (turbidity of ~60 NTU; COD of ~2000 ppm) while being treated with 1) 10 or 20 ppm free chlorine, 2) PAA solution (40 and 80 ppm), 3) UV (10, 20 and 30 mW/cm2), 4) a combination of UV and PAA for 1, 2, and 5 min. Among all the single treatments, the 30 mW/cm2 UV treatment achieved the highest Salmonella reduction on lettuce. For the 2-min treatment group, the 30 mW/cm2 UV treatment achieved 1.98 log reduction, while the 80 ppm PAA and 20 ppm free chlorine resulted in 1.52 and 1.23 log reduction, respectively. The combined treatment of 30 mW/cm2 UV and 80 ppm PAA achieved significantly higher (P