[Generation of GABAergic interneuron-specific PGC-1α knockout mice].

Objective To generate mice which are specific for peroxisomproliferator-activated receptor-γ coactivator-1(PGC-1α) knockout in the GABAergic interneuron. Methods Conditional mice specific for PGC-1αf/+ were introduced from the Jackson Laboratory, USA and initially inbred to obtain homozygote PGC-1αf/f mice. The PGC-1αf/f conditional mice were further crossed with Dlx5/6-Cre-IRES-EGFP transgenic mice to achieve specific knockout of PGC-1α in the GABAergic interneuron. Results The offspring with specific knockout PGC-1α gene were successful for the generation of GABAergic interneuron, with the resulting genotype being PGC-1αf/f; Dlx5/6-Cre-IRES-EGFP. Conclusion The PGC-1αf/f; Dlx5/6-Cre-IRES-EGFP mice were obtained through a proper crossing strategy, which has provided a suitable platform for studying the function of PGC-1α in neuropsychiatric diseases. Key words: Peroxisomproliferator-activated receptor-γcoactivator-1; Conditional gene knockout; Mice; Interneuron