Effects of Rutin on Vegetative Growth of Mung Bean (Vigna radiata)Seedlings and Its Interaction with Indoleacetic Acid

Rutin is one of the natural flavonoids inplants. Effects of rutin and indoleacetic acid (IAA) onvegetative growth of mung bean seedlings were stud-ied and the contents of rutin and IAA in the hypocot-yls of the seedlings were measured. Mung bean seed-lings absorbed rutin through roots, grew more vigor-ously under light at lower rutin concentrations (20–60μg/mL) with longer shoot axis (epicotyl+hypocotyl)and primary root and more lateral roots. The seedlingsexhibited inhibition of elongation of the hypocotylsand radicles when grown in the dark at rutin concen-trations of 20–100 μg/mL or under light at rutin con-centrations of 80–100 μg/mL. Seedlings under lightabsorbed more rutin than those in the dark at rutin con-centrations of 60–80 μg/mL and absorbed less rutin atconcentrations lower than 40 μg/mL or near 100 μg/mL. IAA (0.1 μg/mL) treatment promoted rutin ac-cumulation and inhibited elongation of shoot axis andprimary roots. The rutin content in hypocotyls reachedthe highest level when IAA (0.5 μg/mL) and rutin (40μg/mL) were added to the medium. Rutin repressedendogenous IAA accumulation in hypocotyls of theetiolated seedlings and inhibited absorption of exog-enous IAA from media to seedlings.