Enhancing the Kasugamycin Production by Streptomyces kasugaensis using Olive Mill Waste

The purpose of this study was to produce the kasugamycin by using Streptomyces kasugaensis with olive mill waste containing oil as an energy source. Among the various concentrations of oil, only initial concentration of 20 g/l produced the highest concentration (125.7 mg/l) of kasugamycin. This result indicates that the kasugamycin production was strongly dependent with oil concentrations. Among these fatty acids, only oleic acid (0.1 g/l) produced the highest concentration of kasugamycin 69.7 mg/l, which resulted in 3.4 fold increase in the production than the absence of oleic acid. The corn steep liquor concentration (30 g/l) was found to be the most suitable source for kasugamycin production. Also, fed batch cultures using olive mill waste containing oil and glucose as the sole carbon source were carried out in 51 jar fermentor for seven days. In the case of olive mill waste the maximum kasugamycin concentration was 426.8mg/l, which was about 2.7 fold higher than that of the glucose medium.