Structure-function of anticoagulant TIX-5, the inhibitor of Factor Xa-mediated FV activation.
2021
BACKGROUND The prothrombinase complex consists of factors Xa (FXa) and Va (FVa) on an anionic phospholipid surface and converts prothrombin into thrombin. Both coagulation factors require activation prior to complex assembly. We recently identified TIX-5, a unique anticoagulant tick protein that specifically inhibits FXa-mediated activation of FV. Since TIX-5 inhibited thrombin generation in blood plasma it was concluded that FV activation by FXa contributes importantly to coagulation. OBJECTIVE Here we aimed to unravel the structure-function relationships of TIX-5. METHOD We used a structure model generated based on homology with the allergen Der F7. RESULT TIX-5 was predicted to consist of a single rod formed by several beta sheets wrapped around a central C-terminal alpha helix. By mutagenesis we could show that 2 hydrophobic loops at one end of the rod mediate the phospholipid binding of TIX-5. On the other end of the rod a FV interaction region was identified on one side, while on the other side an EGK sequence was identified that could potentially form a pseudosubstrate of FXa. All 3 interaction sites were important for the anticoagulant properties of TIX-5 in a tissue factor-initiated thrombin generation assay as well as in the inhibition of FV activation by FXa in a purified system. CONCLUSION The structure-function properties of TIX-5 are in perfect agreement with a protein that inhibits the FXa-mediated activation on a phospholipid surface. The present elucidation of the mechanism of action of TIX-5 will aid in deciphering the processes involved in the initiation phase of blood coagulation.
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