The osteoregenerative effects of platelet-derived growth factor BB cotransplanted with mesenchymal stem cells, loaded on freeze-dried mineral bone block: a pilot study in dog mandible.

Dental Research Center, Research Institute of Dental Sciences of Shahid Beheshti University of Medical Sciences, Tehran,IranReceived 15 November 2013; revised 31 January 2014; accepted 13 March 2014Published online 00 Month 2014 in Wiley Online Library (wileyonlinelibrary.com). DOI: 10.1002/jbm.b.33156Abstract: Due to shortcomings associated with autogenousbone graft, the gold standard of craniofacial grafting, investi-gators seek alternatives that are accessible, efficient, andaffordable. Accordingly, in the present pilot study, boneregeneration was induced using bone marrow-derived mes-enchymal stem cells (BMSCs) loaded onto freeze-dried min-eral bone block (FDMBB) in the presence or absence ofrecombinant platelet-derived growth factor-BB (rh PDGF-BB).Eight weeks after the bilateral extraction of premolars of fourmongrel dogs, 25 3 10 mm defects were created at bothsides of the mandible. The right mandible receivedautogenous-BMSC loaded on FDMBB (MSC group), whereasthe left mandible received cellular blocks impregnated withrhPDGF-BB (MSC1PDGF Group). Animals were euthanized 8weeks after grafting. Micro-computed tomography (micro-CT)and histomorphometric analysis demonstrated higher levelsof bone formation for the test group (10.34%60.20 and26.63%63.14, respectively) when compared to the controlgroup (8.20%60.20 and 21.38%65.11). The differences werenot statistically significant (P>0.05). According to the per-formed micro-CT and histomorphometric analysis, adding 0.5mg rhPDGF-BB (0.3 mg/mL) to the combination of BMSC/FDMBB did not significantly increase bone formation insupracrestal defect in dog mandible.