Preparation of chicken gamma-IFN DNA-chitosan nanoparticles for gene expression in vitro.

In present study,the protection of nanoparticles on recombinant plasmid was investigated by evaluating the DNA-chitosan nanoparticles(CNPs) carrying chicken interferon-γ(ChIFN-γ) recombinant plasmid.The nanoparticles were spherical shape with a mean diameter of 1726 nm under the electronic microscope and had the Zeta potential of 20.8 mV ±2.9 mV measured by the Photon Correlation Spectroscopy(PCS).The encapsulation efficiency of DNA was 91.21 %±2.54 % and the DNA content in the nanoparticles was 31.93 %±0.16 %.The capsulated DNA could be protected from the degradation by DNaseⅠ.The transfection efficiency of CNPs was about 16.3% of that the LipofectamineTM 2000 reagent.Our results also showed that CNPs could express successfully in the CEF cells,and were nontoxic to cultured cells.