Rhesus monkey embryonic stem cells differentiation,proliferation and allotransplantation
2013
To investigate the characteristics of rhesus monkey embryonic stem
cells and to promote their clinical application, the differentiation
and proliferation of rosettes neural stem cells from GFP marked rhesus
monkey embryonic stem cells were studied The results showed that: 1) A
stable and high-efficient neural differentiation system was
established. More than 95% of the embryonic stem cells were
differentiated into neural stem cells on the 12th days of
differentiation; 2) the rosettes neural stem cells differentiated from
the rhesus monkey embryonic stem cells could maintain their
rosettes-shape by proliferating with bFGF/EGF; 3) the neural stem cells
could differentiate into neurons after transplanted into the rhesus
monkey brain. In conclusion, the rosettes neural stem cells
differentiated from rhesus monkey embryonic stem cells could maintain
their characteristics after proliferation with bFGF/EGF and they could
survive and differentiate into neurons after transplanted into the
rhesus monkey brain, which strongly supports the clinical application
of neural stem cells in the future.
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