Compatibility of bone marrow mesenchymai stem cells and polylactic glycolic acid scaffold

Objective To study separately the compatibility between bone marrow mesenchymal stem cells （BMSCs） and polylactic glycolic acid （PLGA） scaffolds, or between differentiated neural-like cells and PLGA, and to explore the effects of both composites repairing peripheral nerve injury. Methods （ 1 ） In vitro, BMSCs were cultured by differential adherence, and composited PLGA, via fluorescence mi- croscope, flow cytometry（FCM） and scanning electron microscope was also applied to investigate the information of BMSCs＇ adhesion and induction into neural-like cells in the scaffolds. （2） In vivo, SD rat models with the injury for right sciatic nerve, were randomly divided into 4 groups. Bold scaffold, BMSCs-PLGA complex, and neural-like cells-PLGA complex were separately transplanted into SD rat models, and direct never suture. The ramp test was used to obsvered lower extremity motor function of SD rat models after oper ation. Nerve biopsy specimens was assayed after SD rat were killed in 60 days. Results （ 1 ） In vitro, BM- SCs can be exactly grow on the PLGA. It is have no different in optical density value（OD） compared to ceils inside the culture plates （ P 〉 0. 05 ）. （In vivo, BMSCs and nerve-like cells combined with PLGA, which can restore to peripheral nerve injury, and promote the recovery of lower extremity motor function of SD rat models. After operation for 3 weeks, the angle of ramp test in direct suture groups, bold scaffold groups, BM- SCs-PLGA groups, and neural-like cells-PLGA groups was （38. 32±5.51 ）o, （40. 27±2. 74 ）o, （45.14±3.43 ） o, （60. 56 ±4. 70） 0, and neural-like cells-PLGA groups was significantly higher than other groups. Pathologieal examination showed nerve tissue with the host stand a good biocompatibility. Conclusion BMSCs can be induction into neural-like eells;PLGA have good compatibility with them. Key words: Bone marrow mesenchymal stem cells;  Neural-like cells;  Scaffold material; Compatibility