LC Determination of Clindamycin Phosphate from Chitosan Microspheres

A simple, rapid and precise reverse phase LC method was adopted, modified and validated for the determination of clindamycin phosphate from chitosan microspheres prepared by spray drying method. Separation was performed using ACE5 C18 reversed phase column (150 mm × 4.6 mm, 5 μm) with acetonitrile:phosphate buffer at pH 2.5 (25:75 v/v) as mobile phase. The limit of detection was 46.43 × 10−3 μg mL−1, with UV detection at 210 nm. No interference from chitosan and other excipients was observed. Therefore an incorporation efficiency of microspheres could be determined accurately and specifically.