Culture method for large-scale preparation of clinical treatment level dermal multipotent stem cells for cell transplantation

The invention discloses a culture, screening and amplification technology of clinical treatment level dermal multipotent stem cells. These bottlenecks are always hot and difficult problems of research in the field of biological stem cells; at present, separation and purification are carried out by using the remarkable adhesion characteristic of a basement membrane, and the clinical treatment level dermal multipotent stem cells are obtained by adopting a three-dimensional culture and amplification means. The technology is applicable to the screening and separation of a target cell level by depending on amplifying adherent type cells and providing a three-dimensional high-simulation in-vivo extracellular matrix system. The technology is a novel biological cell culture technology, and adult (embryo skin) stem cells are subjected to in-vitro culture in a high-simulation in-vivo attached environment. The environment where a high-simulation substrate is located adheres, screens and cultures target dermal multipotent stem cells, so that the passage capability is high, the multiplication capacity is high, and a differentiated full-thickness dermal structure can be formed in an in-vitro environment. Immunogenic cells are screened out, so that immunologic escape is safely obtained, the transplantation time is prolonged, the clinical application of tissue engineering is improved, the healing of a surface of wound is promoted, and a clinical treatment level cellular solving scheme with absolute advantages is provided for skin diseases.