Platelet transmembrane potential detection method

The present invention relates to a platelet transmembrane potential detection method, which comprises: taking a washed platelet sample, carrying out re-suspending dilution with Hepes-Tyrodes buffer containing calcium chloride, adding to a flow tube, and recording as F; taking a washed platelet sample, carrying out re-suspending dilution with Hepes-Tyrodes buffer having 120-150 mmol/L of potassium chloride and 2-20 mmol/L of sodium chloride, adding to a flow tube, and recording as F0; adding gramicidin with a final concentration of 0.1-5 [mu]g/L to the F0 flow tube; adding DiBAC4(3) with a final concentration of 10-200 nmol/L to the F flow tube and the F0 flow tube, and dyeing; loading onto the machine and detecting; and calculating the result according to the corrected Nernst equation. According to the present invention, the method has characteristics of less platelet destruction, high sensitivity, good reproducibility, enrichment of the existing platelet storage quality assessment method, and prediction of the platelet storage damage.