PP60C—SRC／ERK1／2信号通路调节大鼠精原干细胞活性

Objective To investigate the effect of PP60C-SRC on the viability of rat spermatogonial stem cells in 9 day-old rat. Methods MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense C-SRC oligodeoxynucleotides (ODNs) and PD98059 in vitro; Western blot was used to observe the PP60C-SRC and phosphorylated extracellular regulated kinasel/2 (p-ERK1/2) contents. Results Compared with that in control group, the viability of the cells was decreased by 8.1% (P＜0.05) and 9.4% (P＜0.01), respectively, after treatment with antisense C-SRC ODNs and PD98059. Compared with the control group, PP60C-SRC and p-ERK1/2 contents were decreased by 33.8% and 38.4% (both P＜0.01), respectively, after transfection of antisense C-SRC ODNs. Compared with the control group, the p-ERK1/2 content was decreased by 34.5% (P＜0.01) in the spermatogonial stem cells after treatment with PD98059. Conclusion PP60C-SRC regulates the viability of rat spermatogonia stem cells through p-ERK1/2.