Application of thin liquid-based cytology and HPV in situ hybridization in the diagnosis of cervical diseases

Objective To explore the screening value of thin liquid-based cytology test(TCT), cervical biopsy and HPV in situ hybridization in the cervical disease, so as to guide clinical applications.Method Four hundred-three cases of cervical disease were TCT checks on;72 of inflammatory response to changes and 227 of more than ASCUS were carried by cervical biopsy under colposcopy routine examination.According to the results of the TCT and histology, 171 cases underwent HPV in situ hybridization.Result TCT results of histology and positive results in line with the rate of change in the inflammatory response, ASCUS, ASCUS-H, LSIL, HSIL and SCC were 95.83%(69/72), 14.84%(23/155), 22.73%(5/22), 78.57%(33/42), 100%(3/3) and 100%(5/5) respectively.In cytology diagnosis, the higher the level, the greater the guide importance.More than LSIL changes as a positive diagnosis of cytology, the positive rate was 12.41%(72/403);more than CIN Ⅰ disease as a positive diagnosis, the rate was 17.39%(52/299), their difference was not significant(P0.05).In HPV in situ hybridization, the rate of infection was 29.82%(51/171).HPV infection rates of chronic inflammation with hyperplasia, CIN, CIN Ⅱ-Ⅲ and SCC were 14.27%(14/98), 37.93%(22/58), 100%(10/10) and 100%(5/5) respectively.The 16/18 high-risk subtypes play an important role in development of cervical cancer and precancerous lesions.Conclusion Whether the TCT, biopsy examination or HPV in situ hybridization is all with the advantages and disadvantages, so the clinical doctor should take into account the condition of the patient and the bearing capacity of the economy, and choice reasonable and effective tests in order to reduce the rate of missed diagnosis and over-diagnosis.