Polymerase Chain Reaction Used for Monitoring multiple Gene Integration in Agrobacterium-Mediated Transformation

Characterizing plants following Agrobacterium-mediated transformation has become an important consideration in the creation of transgenic plants. As an alternative to Southern blot analysis, polymerase chain reaction (PCR) was used to screen for the simultaneous integration of two foreign genes for neomycin phosphotransferase II (NPT II) and β-glucuronidase (GUS) in alfalfa (Medicago sativa L.) transformed with Agrobacterium tumefaciens