Ultrafast Laser Stimulation in Stem-cells and Its Potential for Cell Differentiation Induction

We demonstrate that our multi-photon stimulation system using ultra-short pulsed laser can modulate molecular signaling system such as changes in [Ca2+]i and ROS in mesenchymal stem cells and the expression of early osteoblastic marker could be activated by solely laser illumination in cells 3 days after induction. This project was supported by Research Grants Council (N_CUHK407/16) and National Natural Science Foundation of China (81661168014).