Apoptotic Cardiomyocyte Beating Frequency Detected with Optical Intensity Fluctuation Spectrometer

Dynamical spectroscopy of intensity fluctuations allows measurements of the dynamic scattering of light and motility detection of biomicroparticles in suspensions and tissues. Spectroscopy of intensity fluctuations allows the detection of complex rhythms of the rat heart in vitro. The optical portable spectrometer used detected Fourier spectra and real-time rhythms in rat cardiomyocytes, the beating in the infarct model of the rat heart. The findings indicated that single cardiomyocyte beating was superimposed on the autowave heart tissue rhythm and associated with the apoptotic process. A dependence was observed of the superposition of individual cardiomyocyte beatings at 8–22 Hz on their metabolic state and autooscillations of isolated rat heart tissue at 1–2 Hz.