Effect of Hydrosol Extracted from Chrysanthemum boreale Makino Flower on Proliferation and Migration in Human Skin Keratinocyte

Abstract: In the present study, we extracted the hydrosol from flower of Chrysanthemum boreale Makino (CBMF hydro-sol) by steam distillation and tested the effect of the CBMF hydrosol on skin regeneration using normal human keratino-cytes (HaCaTs). CBMF hydrosol induced proliferation as well as migration in HaCaTs in a dose-dependent manner. Treatment with 1 µ g/mL CBMF hydrosol increased proliferation to 143.71 ± 3.37% and migration to 139.98 ± 5.72% compared with a control group. CBMF hydrosol also significantly enhanced the phosphorylations of extracellular sig-nal-regulated kinase (Erk) 1/2 and serine/threonine-specific protein kinase (Akt) in HaCaTs. Moreover, CBMF hydrosol dose-dependently induced sprout outgrowth in HaCaTs. These results demonstrate that CBMF hydrosol has skin re-generation and wound healing activity in HaCaTs. Therefore, CBMF hydrosol could be used as a potential cosmetic material. Keywords: Chrysanthemum boreale Makino, keratinocyte, proliferation, migration, skin regeneration