Determination of the molar extinction coefficients of the deuteroferriheme analogues of peroxidase enzyme compounds I and II

Absorbance data obtained in the stopped-flow spectrophotometric study of the «in situ» biphasic regeneration of deuteroferriheme (dfh) following its oxidation by NaOCl are utilized to calculate extinction coefficients of species presumed to be involved in series pseudo-first-order redox regeneration processes. Such data are interpreted in terms of a previously proposed mechanism of the type A k1 →B k2 →C where A denotes the dfh-OCl - oxidation product