Effect of decorin gene overexpression on migration and invasion of cholangiocarcinoma cells
2014
Objective To investigate the effects of decorin (DCN) gene overexpression on the migration and invasion of human cholangiocarcinoma cells.Methods The pEGFP-DCN plasmids and pEGFP-N1 control plasmids were transfected into the human cholangiocarcinoma cell line QBC939 cells.The mRNA and protein expression of decorin and E-cadherin in QBC939 cells transfected with plasmid overexpressing decorin was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting respectively.Transwell assay was used to observe the migration and invasion.Results Compared to the control plasmids,RT-PCR showed the mRNA expression of decorin in the pEGFP-DCN plasmids was increased by 32.34 fold.Western blotting showed the protein expression of decorin was increased by 2 fold.Transwell assay showed that the migration and invasion capacity of the QBC939 cells transfected with pEGFP-DCN was decreased by 56.9% and 40.2% respectively as compared with the control cells.The expression level of E-cadherin was significantly increased after DCN transfection into QBC939 cells as compared with the control cells.Conclusion After the over-expression of DCN gene,the migration and invasion capacity of the QBC939 cells was effectively suppressed,which may be associated with vthe elevated E-cadherin expression.
Key words:
Cholangiocarcinoma; Decorin ; Invasion ; Migration
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