5′-Non-Transcribed Flanking Region and 5′-Untranslated Region Play Distinctive Roles in Sulfur Deficiency Induced Expression of SULFATE TRANSPORTER 1;2 in Arabidopsis Roots

: Plants increase sulfate uptake activity under sulfur deficiency (-S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under -S. Though it is known that transcript levels of SULTR1;2 increase under -S largely due to the function of 5'-upstream region, contributions of 5'-non-transcribed flanking region and 5'-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5'UTR of SULTR1;2 in -S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5'-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5'UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under -S relative to +S regardless of presence of the 5'UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5'UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5'-non-transcribed flanking region of SULTR1;2 at positions -2160 to -155 in -S-responsive transcriptional regulation.