Oncostatin M and its receptors mRNA regulation in bovine granulosa and luteal cells

Abstract Oncostatin M (OSM) and its receptor (OSMR) are members of the interleukin-6 family cytokines. Although OSM and OSMR expression was detected in human ovaries, their function and regulation during follicle development, ovulation and luteolysis have not been studied in any species. The aim of the present study was to investigate the levels of OSM and OSMR mRNA in bovine ovaries and the effect of OSM treatment on cultured granulosa cells. OSM mRNA was not detected in granulosa cells obtained from follicles around the time of follicular deviation and from pre-ovulatory follicles, whereas OSMR transcript levels were greater in granulosa cells of atretic subordinate follicles (P  OSMR mRNA increased in granulosa cells of preovulatory follicles, collected at 12 and 24 h after the ovulatory stimulus with gonadotropins (P  OSM mRNA abundance levels were higher at 24–48 h after PGF-induced luteolysis (P  OSMR mRNA was transiently increased at 2 h after PGF treatment (P  BAX/BCL2 mRNA ratio (P  OSMR (P  XIAP mRNA (P