XIAP

1F9X, 1G3F, 1G73, 1I3O, 1I4O, 1I51, 1KMC, 1NW9, 1TFQ, 1TFT, 2ECG, 2JK7, 2KNA, 2OPY, 2OPZ, 2POI, 2POP, 2QRA, 2VSL, 3CLX, 3CM2, 3CM7, 3EYL, 3G76, 3HL5, 3UW4, 3UW5, 4EC4, 4HY0, 4IC2, 4IC3, 4J3Y, 4J44, 4J45, 4J46, 4J47, 4J48, 4KJU, 4KJV, 4KMP, 4MTZ, 4OXC, 4WVS, 4WVT, 4WVU, 5C0K, 5C0L, 5C3H, 5C3K, 5C7A, 5C7B, 5C7C, 5C7D, 5C83, 5C8433111798ENSG00000101966ENSMUSG00000025860P98170Q60989NM_001167NM_001204401NM_001301639NM_001301641NM_009688NP_001158NP_001191330NP_001288568NP_001288570NP_033818X-linked inhibitor of apoptosis protein (XIAP), also known as inhibitor of apoptosis protein 3 (IAP3) and baculoviral IAP repeat-containing protein 4 (BIRC4), is a protein that stops apoptotic cell death. In humans, this protein (XIAP) is produced by a gene named XIAP gene located on the X chromosome.1c9q: AVERAGE NMR SOLUTION STRUCTURE OF THE BIR-2 DOMAIN OF XIAP1f9x: AVERAGE NMR SOLUTION STRUCTURE OF THE BIR-3 DOMAIN OF XIAP1g3f: NMR STRUCTURE OF A 9 RESIDUE PEPTIDE FROM SMAC/DIABLO COMPLEXED TO THE BIR3 DOMAIN OF XIAP1g73: CRYSTAL STRUCTURE OF SMAC BOUND TO XIAP-BIR3 DOMAIN1i3o: CRYSTAL STRUCTURE OF THE COMPLEX OF XIAP-BIR2 AND CASPASE 31i4o: CRYSTAL STRUCTURE OF THE XIAP/CASPASE-7 COMPLEX1i51: CRYSTAL STRUCTURE OF CASPASE-7 COMPLEXED WITH XIAP1kmc: Crystal Structure of the Caspase-7 / XIAP-BIR2 Complex1nw9: STRUCTURE OF CASPASE-9 IN AN INHIBITORY COMPLEX WITH XIAP-BIR31tfq: NMR Structure of an Antagonists of the XIAP-Caspase-9 Interaction Complexed to the BIR3 domain of XIAP1tft: NMR Structure of an Antagonists of the XIAP-Caspase-9 Interaction Complexed to the BIR3 domain of XIAP2opy: Smac mimic bound to BIR3-XIAP2opz: AVPF bound to BIR3-XIAP X-linked inhibitor of apoptosis protein (XIAP), also known as inhibitor of apoptosis protein 3 (IAP3) and baculoviral IAP repeat-containing protein 4 (BIRC4), is a protein that stops apoptotic cell death. In humans, this protein (XIAP) is produced by a gene named XIAP gene located on the X chromosome. XIAP is a member of the inhibitor of apoptosis family of proteins (IAP). IAPs were initially identified in baculoviruses, but XIAP is one of the homologous proteins found in mammals. It is so called because it was first discovered by a 273 base pair site on the X chromosome. The protein is also called human IAP-like Protein (hILP), because it is not as well conserved as the human IAPS: hIAP-1 and hIAP-2. XIAP is the most potent human IAP protein currently identified. Neuronal apoptosis inhibitor protein (NAIP) was the first homolog to baculoviral IAPs that was identified in humans. With the sequencing data of NIAP, the gene sequence for a RING zinc-finger domain was discovered at site Xq24-25. Using PCR and cloning, three BIR domains and a RING finger were found on the protein, which became known as X-linked Inhibitor of Apoptosis Protein. The transcript size of Xiap is 9.0kb, with an open reading frame of 1.8kb. Xiap mRNA has been observed in all human adult and fetal tissues 'except peripheral blood leukocytes'. The XIAP sequences led to the discovery of other members of the IAP family. XIAP consists of three major types of structural elements (domains). Firstly, there is the baculoviral IAP repeat (BIR) domain consisting of approximately 70 amino acids, which characterizes all IAP. Secondly, there is a UBA domain, which allows XIAP to bind to ubiquitin. Thirdly, there is a zinc-binding domain, or a “carboxy-terminal RING Finger”. XIAP has been characterized with three amino-terminal BIR domains followed by one UBA domain and finally one RING domain. Between the BIR-1 and BIR-2 domains, there is a linker-BIR-2 region that is thought to contain the only element that comes into contact with the caspase molecule to form the XIAP/Caspase-7 complex. XIAP stops apoptotic cell death that is induced either by viral infection or by overproduction of caspases. Caspases are the enzymes primarily responsible for cell death. XIAP binds to and inhibits caspase 3, 7 and 9. The BIR2 domain of XIAP inhibits caspase 3 and 7, while BIR3 binds to and inhibits caspase 9. The RING domain utilizes E3 ubiquitin ligase activity and enables IAPs to catalyze ubiquination of self, caspase-3, or caspase-7 by degradation via proteasome activity. However, mutations affecting the RING Finger do not significantly affect apoptosis, indicating that the BIR domain is sufficient for the protein’s function. When inhibiting caspase-3 and caspase-7 activity, the BIR2 domain of XIAP binds to the active-site substrate groove, blocking access of the normal protein substrate that would result in apoptosis. Caspases are activated by cytochrome c, which is released into the cytosol by dysfunctioning mitochondria. Studies show that XIAP does not directly affect cytochrome c. XIAP distinguishes itself from the other human IAPs because it is able to effectively prevent cell death due to 'TNF-α, Fas, UV light, and genotoxic agents'. XIAP is inhibited by DIABLO (Smac) and HTRA2 (Omi), two death-signaling proteins released into the cytoplasm by the mitochondria. Smac/DIABLO, a mitochondrial protein and negative regulator of XIAP, can enhance apoptosis by binding to XIAP and preventing it from binding to caspases. This allows normal caspase activity to proceed. The binding process of Smac/DIABLO to XIAP and caspase release requires a conserved tetrapeptide motif.