Virus-induced gene silencing in tomato.

The tomato phytoene desaturase(PDS) gene fragment was obtained by using RT-PCR from tomato.Tobacco rattle virus vector(pTV00) and PDS fragment were digested by double restriction enzymes and recombinant pTV00-PDS vector was constructed.The constructed vector was transformed into Agrobacterium GV3101 to further inoculate tomato plants.The infected plants showed obvious photobleaching symptom,while semi-quantitative RT-PCR result demonstrated that the decline of PDS mRNA redundancy during infection.The established VIGS system will benefit the high-throughout function analysis of tomato genes in the following experiments.