肾移植受者外周血IL-2 ELISA检测与PBL IL-2 mRNA实时定量PCR检测的比较

Objective To compare the consistency and accuracy of determination of peripheral blood lymphocyte (FBL) interleukin 2 (IL-2) with ELISA and evaluation of the expression of FBL IL-2 mRNA with real time PCR (RT-FCR). Methods In 20 kidney recipients, the FBL IL-2 level was determined with ELISA and the mRNA expression in PBL IL-2 was evaluated by real time fluorescence quantitative PCR (RT-FQ-PCR) before operation and C0/C2 time on the 7th day after operation. The same detection and evaluation were also done in 15 chronic allograft nephropathy (CAN) patients and 5 non CAN patients. Results IL-2 and IL-2mRNA expression showed positive correlation before operation and 1 year later after operation, R^2=0.58 and 0.4 respectively, both P＜0.01. No apparent linear relation was found at C0 and C2 time on the 7th day after operation, R^2=0.15 and 0.08 respectively, both P＞0.05. On the 7th day after operation, IL-2 and the inhibition rate of IL-2 mRNA expression were (16.31±9.59)% and (69.92±7.10)%, respectively. A positive correlation existed between C2 IL-2 concentration and the inhibition rate of IL-2 mRNA expression, R^2=0.56, P＜0.01, while no apparent correlation was found between C2 IL-2 concentration and the IL-2 inhibition rate, R^2=0.02, P＞0.05. Also, IL-2 inhibition and FBL IL-2mRNA inhibition showed no linear correlation. Conclusions The technique of determining FBL IL-2 by ELISA can be used for evaluating immunity status and immunosuppressive tolerance in patients with renal transplantation before the operation and at stable stage after opera non. However, it should not be used for assessing perioperative immunity status and evaluating effect of cyclosporine.