Mucopeptidhydrolasen in Escherichia coli B : II. Quantitative Bestimmungsverfahren und säulenchromatographische Trennung einiger Mucopeptidhydrolasen
2014
A quantitative method for the estimation of cell wall mucopeptides and their enzymatic degradation products by paper chromatography is described. The procedure can be used for measuring the activities of mucopeptidehydrolases as well as for in vivo studies of the metabolism of cell wall mucopeptides.A partial purification of E. coli mucopeptidehydrolases was achieved by column chromatography on DEAE-Sephadex.
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