Fluorescence diffuse optical tomography measurements for tissue engineering
2007
Currents eorts in tissue engineering (TE) are directed towards growing 3D volumes of tissues. In response to TE
needs, we are developing a non-invasive technique based on fluorescence diuse optical tomography (FDOT) to
image in 3D, via fluorescence labelling, the formation of micro-blood vessels in tissue cultures grown on biodegradable
scaolds in bioreactor conditions. In the present work, we use a non-contact FDOT setup developed for
small animal imaging for our measurements. We present experimental results showing the feasability to localize
a fluorophore-filled 500μm capillary immersed in a scattering medium contained in a cylindrically-shaped glass
tube. These conditions are representative of experiments to be carried on real tissue cultures. Time-resolved
scattering-fluorescence measurements are made via Time-Correlated Single Photon Counting (TCSPC) and we
use numerical constant fraction discrimination (NCFD) to obtain primary localization information from our
time-resolved data.
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