Difructose anhydride III promotes absorption of the soluble flavonoid αG-rutin in rats

A highly soluble quercetin glycoside, αG-rutin, is a glucose adduct of insoluble rutin. We examined the effects of difructose anhydride III (DFAIII; di-d-fructofuranosyl 1,2‘:2,3‘-dianhydride) on intestinal absorption of αG-rutin by rat experiments. αG-rutin, rutin, quercetin, and the quercetin conjugate appeared in the portal blood after an intubation of αG-rutin (100 μmol), DFAIII effected higher portal concentrations of αG-rutin in portal cannulated rats. In ligated jejunal and ileal loops of rats, αG-rutin, rutin, quercetin, and the quercetin conjugate appeared in the intestinal mesenteric blood at both 30 and 60 min in both loops; the concentration of αG-rutin was 1.5−3 times higher when absorbed in the presence DFAIII. In the isolated mucosae of the jejunum and ileum, mucosal-to-serosal passage of αG-rutin increased with the addition of 100 μmol of DFAIII. These results indicate that αG-rutin is absorbed as the intact form and that DFAIII stimulates its absorption in the small intestine. Keywords: D...