Addendum to “Regulation of the water channel aquaporin‐1: isolation and reconstitution of the regulatory complex” [Cell Biol. Int. 28(1) (2004) 7–17]*

In a routine assay using atomic force microscopy (AFM) to image red blood cells (RBCs) under various experimental conditions, rapid increase in RBC size following exposure to water (up to a 21% increase in volume), or mastoparan (up to a 73% increase in volume) was demonstrated. The increase in size of RBCs following exposure to water was much lower when assessed by the AFM; however, this modest increase in RBC volume following exposure to water was undetectable by our light-scattering analysis. Although, 150% increase in light-scattering intensity was observed when RBCs were exposed to mastoparan (Fig. 5B; AbuHamdah et al., 2004), little change in light-scattering was detected following exposure to water. Similar to the increase in light-scattering following exposure to mastoparan, we were able to detect an increase in lightscattering when RBCs were exposed to tributyltin chloride (TBT), as previously reported by Ohkuma