In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.

AbstractIn this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side. PRP was loaded onto the composite scaffold using a secondary lyophilization technology to prepare the tissue engineering dressings. AgNPs were characterized using a transmission electron microscope. The morphologies of the composite dressing were examined under a scanning electron microscope. The silver content of the dressing was measured by inductively coupled plasma mass spectrometry. The asymmetric wettability of the composite dressing was demonstrated by water contact angle measurement. Relatively high porosity, favourable moisture retention capability and appropriate tensile strength were observed by measuring the physical and mechanical properties. Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro. The total...