3D bioprinting stem-cells with solubilized tendon extracellular matrix (ECM) based hydrogel

3D bioprinting aims to generate biological structures similar to natural counterparts such as tissues or organs in terms of their functions and morphology [1-2]. The major challenge with this technique is the choice of extra cellular matrix (ECM)-like biomaterial as a cell encapsulating agent. The objective of this research is to develop a composite hydrogel that would provide important biological cues to host cells. A new composite hydrogel system based on a mixture of natural ECM and agarose hydrogel is developed. ECM based hydrogel is derived from bovine native tendon by decellularization and enzymatic digestion procedures. Decellularized and solubilized tendon tissues contain important structural and bioactive extracellular matrix components such as collagen, which serves as anchorage sites for host cells.