아밀로이드 베타로 유발한 알츠하이머병 모델에서 신선초의 기억력 개선 효과

Objectives : Amyloid β (Aβ) could induce cognitive deficits through oxidative stress, inflammation, and neuron death in Alzheimer's disease (AD). This study was investigated the effect of Angelica keiskei KOIDZUMI (AK) on memory in Aβ-induced an AD model. Methods : AK was extracted uses 70% ethanol solvent. Total polyphenol and flavonoids content were obtained by the Folin-Ciocalteu and the Ethylene glycol colorimetric methods, respectively. The antioxidant activities were assessed through free radical scavenging assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) methods. Intracerebroventrical (i.c.v) injection of Aβ 1-42 was used to induce AD in male ICR mice, followed by administrations of 5, 10 or 20 ㎎/㎏ AK on a daily. Animals were subjected to short and long term memory behavior in Y-maze and passive avoidance test. Results : The total polyphenol and flavonoids contents of the AK extract were 88.73±6.36 ㎎ gallic acid equivalent/g, 84.21±5.04 ㎎ rutin equivalent/g, respectively. The assays of DPPH and ABTS revealed that AK extract in treated concentrations (31.25, 62.5, 125, 250, 500, 1000 ㎍/㎖) increased antioxidant activity in a dose-dependent manner. Oral administration of AK extract significantly reversed the Aβ 1-42-induced decreasing of the spontaneous alternation in the Y-maze test and Aβ 1-42-induced shorting of the step-through latency in the passive avoidance test. Conclusions : The findings suggest that AK indicated the antioxidant protective effects against Aβ-induced memory deficits, and therefore a potential lead natural therapeutic drug or agent for AD.