Spectral Analysis for Non-Invasive Total Hemoglobin Measurement in the Region from 400 to 2500nm

Absorption spectra of blood components have been measured for the purpose of predicting the total hemoglobin concentration. We obtained absorption spectra of major blood components from the visible to near-infrared of region. In the near-infrared, water is the main absorbing constituent. The amount of water in the sample cell varies depending on the volume of solute concentration(water displacement). We acquired water-compensated spectra by considering the variation of water volume depending on the variation of analyze concentration. Those spectra show inherent absorption peaks of analyzes and linearity with respect to concentration. We selected wavelengths for non-invasive measurement of hemoglobin concentration considering the scattering effect of tissue and the interference of other blood components.