Cytohistology, colposcopy and in situ hybridization in cervical preneoplastic lesions: A correlative study

Abstract Formalin-fixed, paraffin-embedded cervical tissues from 106 patients which exhibited a colposcopically atypical transformation zone, were examined for the presence of HPV DNA by in situ hybridization using biotin-labeled HPV 6 11 and 16 18 DNA probes. From the comparative study between histologic, cytologic, colposcopic findings and virologic results it is confirmed that HPV 6 11 DNA is detected more frequently in the less severe lesions, whereas HPV 16 18 DNA is predominantly found in the most severe dysplastic lesions.