Conditioned medium preparation of mesenchymal stem cells used for skin aging restoration

The invention discloses a conditioned medium preparation of mesenchymal stem cells used for skin aging restoration. A preparing method of the conditioned medium preparation includes: culturing the mesenchymal stem cells under normal conditions until the growth density of the mesenchymal stem cells is 60-80%, performing liquid changing, culturing with a phenol red-free DMEM, adding astragaloside, performing induction culturing in an incubator for 24 h, removing cytokines having a molecular weight lower than 3000 after the culturing is finished, concentrating, detecting the cytokines in the medium, adding 0.01-0.6 mg/mL of reduced glutathione and 0.01-0.4% of hyaluronic acid into the concentrated culture medium solution. The conditioned medium preparation is rich in a plurality of the cytokines. The astragaloside can maintain the characteristics of the stem cell and stimulates the capability of secreting the plurality of the cytokines of the stem cells. The hyaluronic acid avoids the problems of short half-life period of the cytokines and short curative effect time, and enhances the skin restoration effects.