Changes in NADPH oxidase mRNA level can be detected in blood at inhaled corticosteroid treated asthmatic children

Abstract Aim Oxidative stress, observed in the asthmatic airways, is not localized only to the bronchial system. It would be a great advantage to monitor the oxidative stress markers from blood especially in childhood asthma following the inflammation. Our aim was to measure the levels of antioxidants and the oxidatively damaged biomolecules. We were also interested in the gene expression alterations of the free radical source gp91 phox subunit (CYBB) of the NADPH oxidase system, and the antioxidant heme oxygenase-1 (HMOX-1) isoenzyme in the blood. Our findings were also examined in the context of medical treatment. Main methods Oxidative stress parameters via photometric methods, CYBB and HMOX‐1 expressions via real-time PCR were measured in 58 asthmatic and 30 healthy children. Key findings Higher blood thiobarbituric acid reactive substances (TBARS) (p  Significance Elevated levels of damaged lipid (TBARS) and protein (carbonylated) products corroborate the presence of oxidative stress in the blood during bronchial asthma and suggest the presence of chronic oxidative overload. Our findings also suggest that ICS treatment can influence the relative CYBB mRNA expression in circulating leukocytes in a dose dependent manner.