Role of surface IgM and IgD on survival of the cells from B-cell chronic lymphocytic leukemia

We read with great interest the paper by Bernal et al1 on the ability of anti–human IgM antibodies to prevent spontaneous apoptosis of B-cell chronic lymphocytic leukemia (B-CLL) cells in vitro. These findings suggest that receptor engagement by certain antigens, perhaps autoantigens that are constantly available in vivo, may contribute to the survival of the neoplastic cells. The data, although interesting, are in contrast with previous findings of other groups including our own that showed that exposure to anti-m antibodies causes rapid apoptosis of certain B-CLL cells.2-4 More recently we have also demonstrated that when an anti–Ig–d chain antibody is used, prolonged cell survival instead of apoptosis is observed.5 From these findings it appears that the specificity of the antibodies to surface Ig plays a crucial role in the outcome of the experiments. Unfortunately, in the experiments by Bernal et al1 very little attention is paid to the specificity of the reagent used. If the anti-Ig reagent (a polyclonal goat anti-IgM antibody) that they used also reacted with light chains then the cells could have been stimulated via surface IgM and IgD. In these conditions, the prevailing physiological pathway would be survival or apoptosis depending on which of the 2 signals is more potent. Differences of about 15% in the Annexin-V staining are barely significant with the presently used methods, according to the experience accumulated by several groups including our own. Thus, 3 B-CLL groups can be distinguished in the work of Bernal et al1(Tab 1): those that do not respond at all to anti-IgM treatment (cases 31, 86, 89); those that barely respond (cases 47, 69, 72, 96, 104, 106, 108, 111, 114, 121); and those who definitely respond (cases 58, 78). The conclusions of the authors, as mentioned in the title of their article, are based primarily upon the latter 2 cases. Bernal et al state that for our previous work we selected for cases expressing CD38. These cases might represent a special subset of B-CLL. CD381 B-CLL cells represent 30% to 60% of all the B-CLL (depending upon the different cohorts studied).6 Even before CD38 became a fashionable marker, it was already known that the cells from only about half of the B-CLL cases responded to anti-Ig stimulation in vitro as assessed in Ca11 mobilization or tyrosine kinase phosphorylation assays.7,8 We just pointed out that a correlation existed between CD38 expression and viability of the signal transduction pathway. Based upon these considerations the question arises of how many of the cases utilized by Bernal et al had a functional IgM/IgD-dependent signal transduction pathway initiated by surface Ig cross-linking. This is not trivial, considering the general implications of the paper brought about by their title. A final comment concerns the observation reported in the paper that certain genes potentially related to antiapoptotic activity are up-regulated following exposure of B-CLL cells to anti-IgM antibodies. This up-regulation (which is not very dramatic as apparent from the figures shown by Bernal et al) should not inevitably be taken as evidence for the fact that anti-IgM stimulation causes activation of an antiapoptotic program. In studies that we have not published, we have compared the capacity of anti–m chain or anti–d chain stimulation to promote the synthesis of a variety of molecules (M.M. and S.Z., unpublished data, May 2001). There is virtually comparable up-regulation of the same molecules studied by Bernal et al following exposure to anti-d or anti-m reagents. However, the 2 types of stimuli had a remarkably different effect on caspase activation and consequently on apoptosis. In conclusion, the interesting hypothesis proposed by Bernal et al does not appear to be sustained by the available experimental data. It is possible that B-CLL cells are exposed to a variety of apoptotic and antiapoptotic signals in vivo and that the survival of a large fraction of them depends upon the fact that the antiapoptotic signals are prevailing.