Induction of Rhesus Monkey Embryonic Stem Cells into Hepatocyte-like Cells by a Three-step Method
2010
Embryonic stem cells (ESCs) hold great potential for the treatment of
liver diseases. Here, we reported the induction of rhesus monkey ESCs
along a hepatocyte lineage by a three-step method. The Rhesus monkey
ESCs were suspended in DMEM medium supplemented with 10% FBS for 11
days to form embryonic bodies (EB) which contained early endoderm or
hepatic cells by expression of early hepatic genes. EB of 11 days were
transferred into ECM coated dishes and cultured with aFGF, BMP-4 and
OSM at different time points for further studies. After induction by
aFGF and BMP-4 for 7 - 10 days, the differentiated cells showed a
hepatocyte-like morphology, expressed hepatic marker genes (such as
AFP,ALB APOH,G-6-P and TAT) and proteins (AFP,ALB
and CK18), and possessed the capacity to storage intracytoplasmic
glycogen. After further induction with OSM for 7 - 10 days, some cells
shared similar characteristics of matured hepatocytes, such as
expression of genes of CYP1B1 and ADH1C, and ICG uptaken, indicating
the presence of functional hepatocytes in the differentiated cells.
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