Inhibiting effect of Endostar combined with ginsenoside Rg3 on breast cancer tumor growth in tumor-bearing mice

Abstract Objective To study the inhibiting effect of Endostar combined with ginsenoside Rg3 on breast cancer tumor growth in tumor-bearing mice. Methods Female mice were selected as experimental animals, and breast cancer tumor-bearing mouse models were established and then divided into groups A, B, C and D that respectively received saline, recombinant human endostatin, ginsenosides Rg3 and recombinant human endostatin combined with Rg3 intervention; 7 d, 14 d and 21 d after intervention, tumor tissue volume was measured; 21 d after intervention, mice were killed, tumor tissue was collected, and mRNA contents of angiogenesis molecules, invasion molecules, autophagy marker molecules and autophagy signaling pathway molecules were detected. Results At 7 d, 14 d and 21 d after intervention, tumor tissue volume of groups B, C and D was lower than that of group A, and tumor tissue volume of group D was lower than that of groups B and C; mRNA contents of VEGFA , VEGFB , VEGFC , MMP2 , MMP9 , p62 , mTOR , PI3K , Akt , JNK and Beclin-1 in tumor tissue of groups B, C and D were significantly lower than those of group A, and LC3-II / LC3-I was significantly higher than that of group A; mRNA contents of VEGFA , VEGFB , VEGFC , MMP2 , MMP9 , p62 , mTOR , PI3K , Akt , JNK and Beclin-1 in tumor tissue of group D were significantly lower than those of groups B and C, and LC3-II / LC3-I was higher than that of groups B and C. Conclusions Endostar combined with ginsenoside Rg3 has stronger inhibiting effect on breast cancer tumor growth in tumor-bearing mice than single drug, and it can inhibit angiogenesis and cell invasion, and enhance cell autophagy.