Abstract 19265: Hyperoxic Cycling of Human Inducible Pluripotent Derived Cardiomyocytes (hipsc-cms) Augments Transplanted Cell Survival in Rats Subjected to Myocardial Infarction

Introduction: Cardiac regeneration via stem cells following acute myocardial necrosis or apoptosis is a promising therapy. However, low cell survival remains a significant limitation primarily due to the hypoxic microenvironment of the ischemic heart. Hypothesis: Preconditioning of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) with hyperoxic oxygen cycling will enhance cell resistance to hypoxic stress and improve cell survival upon transplantation into the ischemic heart. Methods: Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) were subjected to two cycles of hypoxia and reoxygenation as mentioned below. Experiments were performed in three different groups in vitro. 1) HYP (Hypoxia only, 12 h); 2) HP-21 (2 cycles of Hypoxia (15 min) and reoxygenation (21% O2, 30 min) followed by 12 h of continuous hypoxia; and 3) HP-95 (2 cycles of Hypoxia (15 min) and reoxygenation (95% O2, 30 min) followed by 12 h continuous hypoxia. For in vivo studies hiPSC-CMs (red flu...