ENHANCEMENT OF ENTRAPMENT EFFICIENCY OF POROUS CHITOSAN SCAFFOLD FORMULATION BY THE ADDITION OF PEG 4000

MATERIALS AND METHODS POROUS CHITOSAN SCAFFOLD PREPARATION Briefly different concentration of chitosan and PEG 4000 were dissolved in 0.2 M glacial acetic acid and mixture of solution was poured into stainless steel mould and mould was stored in deep freezer at – 70C for 5 days. After 5 days, mould was placed in lyophiliser (Martin Christ alpha 1-2 LD plus, Germany) for 3 days. After 3 days, blank porous chitosan scaffold was prepared. Gliclazide (GLZ) drug (0.1%) was dissolved in ethanol and poured into porous matrix scaffold with the help of pipette using a post seeding method and drug loaded porous chitosan scaffold formulation was prepared [1].