Method for integrating focusing and detection of cells and miniaturized system thereof

The invention discloses a method for integrating focusing and detection of cells and a miniaturized system thereof. The miniaturized system comprises a microfluidic chip, a data acquisition card, a miniature computer and a sample injection device, wherein the microfluidic chip is formed by a flow channel layer, a substrate layer and a PCB (Printed Circuit Board) which are aligned and packaged in sequence; the flow channel layer is provided with an asymmetric sinusoidal flow channel, a detection main flow channel, polyelectrolyte gel and a conductive-fluid storage pool; the polyelectrolyte gel, the conductive-fluid storage pool and a silver-silver chloride wire form a detection electrode; the silver-silver chloride wire is connected with the data acquisition card and the miniature computer by a transimpedance amplifier and a differential amplifier so as to form a differential impedance detection circuit of the cells; the miniature computer is used for realizing generation of pseudorandom excitation signals, processing of system response signals and analysis and display of multi-performance parameters of the cells. The method and the miniaturized system disclosed by the invention integrate the functions of focusing and detection of the cells, realizes miniaturization and portability of the system and can be widely used for biological study of blood cells and rare cells.