Study on Cell Adhesion Detection onto Biodegradable Electrospun PCL Scaffolds

The polycaprolactone (PCL) known for its slow biodegradability shows potential applications in the fleld of skin scafiold production. In the current study PCL mats with micro scaled structure were produced via electrospinning and treated with NaOH solution for enhanced cell adhesion. The scafiolds were evaluated as an e‐cient stem cell growing material by human skin keratinocytes and flbroblasts culture. The cell viability assay (CellTiter-Blue) of the as spun scafiolds was compared with previously prepared PU electrospun scafiolds, flbrin scafiolds and amniotic membrane scafiold. Signiflcant difierence in cell adhesion was evident between the natural and synthetic scafiolds. As far as the electrospun scafiolds were concerned the signiflcance between, as well as for the NaOH treated, samples was less evident. The repetition of difierent donor cells ∞uorescence within the same scafiold material was also present.